FastQCFastQC Report
Fri 7 Dec 2018
HGHC5BGX9_n01_AHm07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHC5BGX9_n01_AHm07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19874169
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT459180.23104362250315974TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC79700.035.4310246-47
TATGCCG81200.034.7763348-49
CTCGTAT80050.034.38582644-45
TCGTATG75350.032.74836744-45
TATCTCG86900.027.5210440-41
ATCTCGT100150.027.22373642-43
TCTCGTA90700.026.23718642-43
CCGTCTT129750.021.96497352-53
GCTATCT112450.021.52128838-39
AGCTATC128150.021.51627738-39
ATGCCGT118650.021.3176748-49
GTATGCC119600.020.83072746-47
CTATCTC137900.019.89174540-41
GCCGTCT126500.019.86332550-51
TGCCGTC148350.019.54721650-51
TACCGTA21900.019.0860989
GAAGCTA159700.018.23229436-37
CGTCTGA174950.016.73794416-17
CACGTCT179000.016.22649614-15
AAGCTAT156650.016.08572636-37
Produced by FastQC (version 0.11.5)