FastQCFastQC Report
Fri 7 Dec 2018
HGHC5BGX9_n01_AHm06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHC5BGX9_n01_AHm06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28757614
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT539000.18742862325087192TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC94500.033.1738846-47
TATGCCG95700.032.98134248-49
CTCGTAT99550.030.98989944-45
TCGTATG91450.030.41063344-45
TATCTCG108650.024.67824240-41
TCTCGTA115800.023.40072642-43
ATCTCGT132350.023.05852142-43
CCGTCTT166700.019.10526352-53
AGCTATC163700.018.6715338-39
GCTATCT145850.018.64442838-39
GTATGCC157000.018.09196346-47
TACCGTA27950.017.8437129
ATGCCGT157200.017.82726748-49
CTATCTC177550.017.41567440-41
GCCGTCT163500.017.40179350-51
TGCCGTC194600.016.59781650-51
GAAGCTA211200.015.3156536-37
CACGTCT220900.014.68603614-15
CGTCTGA229200.014.23713116-17
ATCGGAA393350.014.17711452
Produced by FastQC (version 0.11.5)