FastQCFastQC Report
Fri 7 Dec 2018
HGHC5BGX9_n01_AHm05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHC5BGX9_n01_AHm05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29457885
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT350540.1189970019911477TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC76450.029.23275846-47
TATGCCG76500.029.21353148-49
TCGTATG72300.027.85567944-45
CTCGTAT79850.026.91719844-45
TATCTCG86350.022.77309240-41
TCTCGTA94300.020.80289642-43
ATCTCGT109000.019.60972442-43
CCGTCTT139500.016.42901452-53
ATGCCGT130950.015.77864148-49
TACCGTA29750.015.64660559
GCTATCT128100.015.40658138-39
GTATGCC138950.014.95571446-47
AGCTATC145600.014.84343338-39
GCCGTCT144800.014.5154450-51
TGCCGTC168050.013.77912250-51
CTATCTC163200.013.6646840-41
CTACCGT38950.013.5361948
CACGTCT201650.011.60082914-15
CGTCTTC184250.011.58805752-53
ACGTCTG181150.011.52395514-15
Produced by FastQC (version 0.11.5)