FastQCFastQC Report
Fri 7 Dec 2018
HGHC5BGX9_n01_AHm04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHC5BGX9_n01_AHm04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27486188
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT788890.28701324461580485TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC131500.039.37163546-47
TATGCCG134550.038.39072848-49
CTCGTAT133600.037.72180644-45
TCGTATG129150.034.27717644-45
ATCTCGT154850.032.19247442-43
TATCTCG143400.029.91017740-41
TCTCGTA147600.029.10752942-43
CCGTCTT186500.027.74801652-53
AGCTATC199050.025.17502238-39
GCTATCT172150.025.06671738-39
GTATGCC185000.024.42979446-47
GCCGTCT185050.024.19202650-51
ATGCCGT185400.024.1335948-49
TGCCGTC216450.024.11682350-51
CTATCTC228600.022.38831540-41
GAAGCTA244050.021.86634636-37
CGTCTTC211900.021.22768252-53
AGTCACC267900.019.84870528-29
TACCGTA30300.019.4382469
GACATAA166550.019.4215035
Produced by FastQC (version 0.11.5)