FastQCFastQC Report
Fri 7 Dec 2018
HGHC5BGX9_n01_AHm03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHC5BGX9_n01_AHm03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15539219
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT347670.22373711317151784TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC54550.037.4851346-47
TATGCCG55800.036.77321248-49
CTCGTAT57650.034.3159744-45
TCGTATG54150.033.5954944-45
ATCTCGT68500.028.5685842-43
TATCTCG64750.027.06877540-41
TCTCGTA65300.026.65893742-43
CCGTCTT88150.023.30504852-53
GCTATCT77350.022.56723438-39
AGCTATC89550.021.85309638-39
ATGCCGT84550.021.82526248-49
GTATGCC85150.021.78296746-47
GCCGTCT85950.021.30400550-51
TGCCGTC103350.020.06118250-51
CTATCTC107250.018.75590940-41
CGTCTTC101300.018.24006552-53
GAAGCTA115200.018.22445936-37
GACATAA91400.017.5132085
CACGTCT123100.017.11255314-15
ATCGGAA216250.016.9575372
Produced by FastQC (version 0.11.5)