FastQCFastQC Report
Fri 7 Dec 2018
HGHC5BGX9_n01_AHm02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHC5BGX9_n01_AHm02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17517106
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT242590.13848748760211876TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG43950.030.58524548-49
CGTATGC44800.030.05804346-47
CTCGTAT46850.028.48925844-45
TCGTATG42700.028.25466744-45
TATCTCG55850.021.3042640-41
TCTCGTA57850.020.6499542-43
ATCTCGT65150.020.19527442-43
TACCGTA18150.016.4870669
GCTATCT73700.016.3055338-39
CCGTCTT85750.016.06379952-53
ATGCCGT78350.015.67133348-49
AGCTATC86900.015.24987838-39
GTATGCC81650.015.2125246-47
GCCGTCT83000.014.90777350-51
CTATCTC97000.013.735455540-41
TGCCGTC101650.013.71462350-51
CTACCGT24550.013.1563948
GAAGCTA117950.012.06098336-37
CACGTCT118900.011.92454714-15
CGTCTGA121600.011.64024516-17
Produced by FastQC (version 0.11.5)