FastQCFastQC Report
Fri 7 Dec 2018
HGHC5BGX9_n01_AHm01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHC5BGX9_n01_AHm01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29092494
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT592810.20376733600082553TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG103700.034.23883448-49
CGTATGC105600.033.62279546-47
CTCGTAT109550.031.58668544-45
TCGTATG100800.031.43056744-45
TATCTCG118000.025.9433240-41
ATCTCGT134850.025.30828742-43
TCTCGTA124100.024.61081942-43
CCGTCTT168900.021.17640552-53
AGCTATC168300.020.41916538-39
GCTATCT149900.020.35893238-39
ATGCCGT159900.020.05126848-49
GTATGCC164050.019.6308946-47
GCCGTCT168550.019.31810450-51
TGCCGTC198150.018.30209450-51
CTATCTC190850.018.14345640-41
GAAGCTA227850.016.27081336-37
TACCGTA28350.016.2518149
CACGTCT229600.015.98125614-15
ATCGGAA406800.015.7985212
CGTCTTC212250.015.51978352-53
Produced by FastQC (version 0.11.5)