FastQCFastQC Report
Fri 5 Feb 2016
HGH7MBCXX_l02n02_t24-d.352000000410e9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGH7MBCXX_l02n02_t24-d.352000000410e9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14999194
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG532560.3550590785078185Illumina Single End PCR Primer 1 (100% over 51bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG82800.036.1387218
CACACGT70500.034.6890119
AGCACAC95200.034.33840617
ACACGTC62250.033.57591220
GTCGCCG108750.032.86060345
AGAGCAC109500.032.3608115
GAGCACA108000.032.14353616
GGTCGCC116300.030.630744
CACGTCT54450.030.4110521
GTGGTCG125300.028.12342
TGGTCGC129050.027.29053543
ACGTCTG49050.026.97135722
CGTCTGA42650.026.58740623
GATCGAG27700.025.2612748
AGATAGA115300.025.1925187
GGTGGTC144650.024.51520741
TCGCCGT31400.024.29541645
CTCGGTG144350.023.92690538
CGGTGGT149700.023.58283840
GATAGAT129300.022.7780088
Produced by FastQC (version 0.11.2)