FastQCFastQC Report
Fri 5 Feb 2016
HGH7MBCXX_l02n02_t23.352000000410cf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGH7MBCXX_l02n02_t23.352000000410cf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9753583
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG324620.33282128218932466Illumina Single End PCR Primer 1 (100% over 51bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG92700.039.85092518
CACACGT84850.038.55294419
AGCACAC105900.038.34654217
AGAGCAC118200.037.32564515
ACACGTC71600.037.29775220
GAGCACA115900.037.1732316
CACGTCT63050.034.6480721
GTCGCCG57050.034.63464745
GGTCGCC57750.034.2153644
CGTCTGA37450.032.08148223
ACGTCTG47700.031.97984122
TGGTCGC64200.030.49739843
AAGAGCA154400.030.32298514
GTGGTCG67250.029.07749742
CTCGGTG74300.025.6200538
GGTGGTC79250.024.70109241
CGGTGGT82450.023.57842440
CGGGGAC5700.022.9012091
TCGGTGG84750.022.72544739
TCTCGGT87650.021.79441837
Produced by FastQC (version 0.11.2)