FastQCFastQC Report
Fri 5 Feb 2016
HGH7MBCXX_l02n02_t22.352000000410a6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGH7MBCXX_l02n02_t22.352000000410a6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18060174
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG573470.317532931853259Illumina Single End PCR Primer 1 (100% over 51bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG76850.035.8925118
GTCGCCG96700.034.30182645
CACACGT71150.033.48715619
GGTCGCC99000.033.41399444
AGCACAC92700.033.3718417
AGAGCAC105150.033.07938415
GAGCACA106200.031.84136816
ACACGTC60100.031.82011220
TGGTCGC112100.029.2081243
GTGGTCG116150.028.20670142
CACGTCT54250.027.53756721
CTCGGTG117350.027.41828338
TCGGTGG135350.023.95436339
GGTGGTC138700.023.71691541
ACGTCTG48950.023.2574622
CGTCTGA40550.023.02616923
AAGAGCA167900.022.80689214
CGGGGAC11600.022.3124581
GATCTCG529900.022.01917645
CGGTGGT149650.021.84615540
Produced by FastQC (version 0.11.2)