FastQCFastQC Report
Fri 5 Feb 2016
HGH7MBCXX_l02n02_t14-d.3520000004108c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGH7MBCXX_l02n02_t14-d.3520000004108c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12945957
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG648140.5006505119706485Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG111500.036.48189518
CACACGT100300.034.88054719
ACACGTC86950.034.64693520
GTCGCCG119950.033.54491845
GGTCGCC119050.033.53348544
AGCACAC132400.033.52686317
AGAGCAC144750.032.85805515
CACGTCT71450.032.81098621
GAGCACA143200.032.67940516
TGGTCGC126700.030.99368943
ACGTCTG60000.030.74891922
GTGGTCG128100.030.6701642
CGTCTGA52100.030.61792623
GGTGGTC144000.027.34405541
CGGTGGT149400.025.85842140
AAGAGCA196300.025.68485314
TCGCCGT32000.025.45763645
CTCGGTG153650.024.5282338
TCTCGGT161650.023.31424937
TCGGTGG165600.022.88007239
Produced by FastQC (version 0.11.2)