FastQCFastQC Report
Fri 5 Feb 2016
HGH7MBCXX_l02n02_t13.35200000041062.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGH7MBCXX_l02n02_t13.35200000041062.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6094636
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG66930.10981787919737947Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG23500.033.7961418
CACACGT24000.030.09220719
AGCACAC30100.028.85234317
AGAGCAC34700.028.33428415
GAGCACA33750.028.13189316
ACACGTC20550.027.6993120
GGTCGCC15050.027.21237444
GTCGCCG15700.025.79930145
CACGTCT17300.025.62007521
CGGGGAC3950.022.2217081
GTGGTCG18600.022.01698942
ACGTCTG15450.021.69827322
CTCGGTG18800.021.30314838
TGGTCGC19650.020.84204743
CGGGGCG1850.020.6817421
CGTCTGA14200.020.4394323
CGGGGTG4650.020.3285451
CCGGGTG4550.019.2913741
AAGAGCA55950.018.49771114
ATCTCGG22350.018.02009836
Produced by FastQC (version 0.11.2)