FastQCFastQC Report
Fri 5 Feb 2016
HGH7MBCXX_l02n02_t12.35200000041049.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGH7MBCXX_l02n02_t12.35200000041049.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7323129
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG211820.28924794305822005Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG32900.034.80794518
CACACGT30650.032.5184419
GGTCGCC39900.032.20523544
GTCGCCG39850.032.1887345
AGCACAC40100.031.81230517
GAGCACA44300.030.8785116
AGAGCAC45350.030.70929315
ACACGTC27100.029.72146620
GTGGTCG46600.027.23403242
TGGTCGC47350.026.9004643
CACGTCT22550.025.74125321
CTCGGTG51050.023.66816938
GGTGGTC55150.023.17335741
ACGTCTG18600.022.86184722
CGGGGCG2450.022.0482271
CGTCTGA15800.021.78696623
TCTCGGT56350.021.48140137
CGGGGAC4750.021.3229561
CGGTGGT59800.021.22076840
TCGGTGG58300.021.1492639
Produced by FastQC (version 0.11.2)