FastQCFastQC Report
Fri 5 Feb 2016
HGH7MBCXX_l02n02_t11.3520000004102f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGH7MBCXX_l02n02_t11.3520000004102f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5921281
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG237380.40089298244754806Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG32900.036.175318
CACACGT31600.033.81885519
AGCACAC38700.032.6721517
ACACGTC26550.032.37063220
GTCGCCG47450.031.77505345
GGTCGCC47900.031.47707644
GAGCACA42150.031.06545816
AGAGCAC43050.030.99088115
CACGTCT22550.030.43026221
TGGTCGC50850.029.65096943
GTGGTCG52650.028.59234442
ACGTCTG20500.026.99880822
CGTCTGA17100.026.97249423
CTCGGTG55950.025.93872838
GGTGGTC60250.024.98398841
CGGTGGT61450.024.34963640
TCGGTGG61600.023.8148939
TCTCGGT61400.023.5624637
TCGCCGT13850.023.07205845
AAGAGCA61400.022.60836614
Produced by FastQC (version 0.11.2)