FastQCFastQC Report
Fri 5 Feb 2016
HGH7MBCXX_l02n02_plasmid-wt.3520000004113b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGH7MBCXX_l02n02_plasmid-wt.3520000004113b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13028039
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG586670.45031335874877254Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG86500.038.0781318
CACACGT76550.035.97384619
GTCGCCG102250.035.89505445
AGCACAC106000.035.25448617
GAGCACA115000.035.25393316
AGAGCAC118700.035.0079615
GGTCGCC104850.034.87604544
ACACGTC65950.033.3978520
TGGTCGC112900.031.89101443
GTGGTCG114450.031.43667642
CTCGGTG108700.031.2770338
CACGTCT54850.030.88651821
ACGTCTG45200.028.1737922
TCGGTGG121550.028.13655539
GGTGGTC130500.027.50051341
CGTCTGA37000.027.12066823
TCTCGGT125100.027.0866337
CGGTGGT132200.026.73819240
AAGAGCA169950.026.58233514
TCGCCGT22250.022.85716645
Produced by FastQC (version 0.11.2)