FastQCFastQC Report
Fri 5 Feb 2016
HGH7MBCXX_l02n02_plasmid-d.35200000041104.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGH7MBCXX_l02n02_plasmid-d.35200000041104.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13552802
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG89150.037.75394418
CACACGT86600.036.1117919
ACACGTC73250.035.59818620
AGCACAC106000.034.17213417
GAGCACA113100.033.81725716
AGAGCAC116800.033.42017415
CACGTCT64250.032.5658121
ACGTCTG53200.028.54666722
CGTCTGA44950.027.0788923
GGTCGCC21000.026.25426344
AAGAGCA160650.025.26433614
GTCGCCG21850.025.12975345
CGGGGAC10550.023.4661221
CTCGGTG23650.022.2626538
CGGGGAG10000.020.0304681
AGATCGG226600.018.4188847
GATCGGA229450.018.219528
TGGTCGC30200.018.10724343
TAGATCG60050.018.0221486
CAGATCG70400.017.641726
Produced by FastQC (version 0.11.2)