FastQCFastQC Report
Fri 5 Feb 2016
HGH7MBCXX_l02n01_t24-d.351000000410ec.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGH7MBCXX_l02n01_t24-d.351000000410ec.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14999194
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC808120.5387756168764801TruSeq Adapter, Index 6 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC125600.030.95645145
TATCTCG126850.029.5163640
CTCGTAT134050.028.9718943
TCGTATG140550.027.61574444
ATCTCGT137650.027.33155641
TCTCGTA145400.026.0451542
ATATCTC181600.020.84059339
GCCAATA536300.019.49673845
AATATCT214350.017.72977638
CAATATC237200.016.1546337
CGCCAAT803150.015.6103145
CCAATAT326200.013.3128345
ACGCCAA1084650.011.72076345
CCCTACG15850.011.64084640
TGCCCCG45150.010.9140965
GTATGCC32500.010.59266345
GTGCCCC47800.010.5918884
TGTAGGG19200.010.4311151
CACGCCA1229050.010.32725844
GTGACTG22300.09.9901561
Produced by FastQC (version 0.11.2)