FastQCFastQC Report
Fri 5 Feb 2016
HGH7MBCXX_l02n01_t23.351000000410c2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGH7MBCXX_l02n01_t23.351000000410c2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9753583
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC392040.401944598205603TruSeq Adapter, Index 5 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT71200.028.85393343
CGTATGC72600.028.69985445
GATCTCG69150.028.56997940
ATCTCGT73550.026.86124241
TCGTATG78200.026.529844
TCTCGTA75950.026.16083742
GTGATCT85000.023.13662738
TGATCTC87250.022.56577139
AGTGATC89750.021.93719937
ACAGTGA210750.015.72680645
CAGTGAT130250.015.39267936
CCCTACG16700.013.20435440
CACAGTG280750.013.17611445
CGGCCTT17350.012.57991245
CTACGGC18150.011.90181742
TACGGCC19600.010.90640843
CCTACGG20350.010.72559841
ACGGCCT20750.010.19341144
ACACAGT365500.010.13938345
TCCCTAC23450.09.6913939
Produced by FastQC (version 0.11.2)