FastQCFastQC Report
Fri 5 Feb 2016
HGH7MBCXX_l02n01_t22.351000000410a9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGH7MBCXX_l02n01_t22.351000000410a9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18060174
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC696370.3855832175260327TruSeq Adapter, Index 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC117850.029.32682845
GATCTCG111000.029.08827240
CTCGTAT117850.028.84966343
ATCTCGT117900.027.40536741
TCGTATG130500.026.57031844
TCTCGTA124800.026.12534742
CGATCTC127050.025.34276439
ACGATCT133300.024.32331838
CACGATC146400.022.17758637
ATCACGA540000.021.2135245
TCACGAT257900.017.55416145
CATCACG839600.016.69625545
CCCTACG29850.013.34190840
CTACGGC31550.013.05148642
CGGCCTT32000.012.86780745
TACGGCC32000.012.6569343
ACATCAC1147650.012.25980744
ACGGCCT33550.012.13921744
CCTACGG34250.011.89085441
TCACGAA51750.011.08749145
Produced by FastQC (version 0.11.2)