FastQCFastQC Report
Fri 5 Feb 2016
HGH7MBCXX_l02n01_t14-d.3510000004108f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGH7MBCXX_l02n01_t14-d.3510000004108f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12945957
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC841980.6503806555204841TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC127600.032.25241545
CTCGTAT131600.031.05006243
TCGTATG134750.030.57469244
AATCTCG132350.030.22759840
TCTCGTA133550.030.0574342
ATCTCGT134800.029.76178441
GAGCGTC47400.028.90643316
AGCGTCG43450.028.0133317
AGAGCGT56300.026.61468915
AAGAGCG57200.026.5892614
GCGTCGT38350.024.46402518
GTAATCT174800.022.8482438
TAATCTC176150.022.77532239
TGTAATC177350.022.60853237
CGTCGTG38500.019.57678419
TTGTAAT237850.017.07562436
CTTGTAA281200.014.739335
CCTTGTA287800.014.362308534
GTCGTGT29250.014.3068620
GTGACTG35500.014.26199151
Produced by FastQC (version 0.11.2)