FastQCFastQC Report
Fri 5 Feb 2016
HGH7MBCXX_l02n01_t13.35100000041065.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGH7MBCXX_l02n01_t13.35100000041065.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6094636
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC88110.14456974953057083TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG19900.020.69118940
CTCGTAT22950.018.82451643
ATCTCGT22450.018.54186841
CGTATGC24300.018.14894945
TCGTATG25200.017.41162544
TCTCGTA25050.016.97693642
GAGCGTC8350.016.43609816
GCTTATC27400.015.35601337
AGAGCGT10750.014.65024815
AGCGTCG8500.014.0286517
TAGCTTA32200.013.62604235
AAGAGCG12250.013.04000214
AGCTTAT33850.012.76243636
TTATCTC37400.011.49077239
CTAGCTT39350.011.32160234
CTTATCT38350.011.20612338
GCGTCGT8900.010.617418
CACTAGC41600.010.49290832
ACTAGCT41900.010.471477533
AGTCACT47400.09.30382529
Produced by FastQC (version 0.11.2)