FastQCFastQC Report
Fri 5 Feb 2016
HGH7MBCXX_l02n01_t12.3510000004104c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGH7MBCXX_l02n01_t12.3510000004104c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7323129
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC263770.36018756463254986TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTCG45050.027.8693540
CGTATGC48050.027.58208845
CTCGTAT48150.027.33806643
ATCTCGT50300.025.18504341
TCTCGTA51100.024.87916442
TCGTATG54200.024.53558544
AGATCTC57300.022.02904739
CAGATCT65400.019.50710338
TCAGATC68450.018.44067837
GAGCGTC10150.017.95464316
AGAGCGT12150.015.9250215
CGATCAG82900.015.9053234
ATCAGAT83700.015.37693836
GATCAGA87050.015.17290135
AGCGTCG10500.014.35642417
AAGAGCG14250.013.89394714
CACGATC106000.012.41793332
GTCACGA109450.012.14985430
AGTCACG111550.011.88045829
ACGATCA112650.011.82469333
Produced by FastQC (version 0.11.2)