FastQCFastQC Report
Fri 5 Feb 2016
HGH7MBCXX_l02n01_t11.35100000041022.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGH7MBCXX_l02n01_t11.35100000041022.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5921281
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC308550.5210865689366879TruSeq Adapter, Index 8 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC51250.030.99590145
CTCGTAT53500.029.56691443
ATCTCGT52550.029.41682441
TCTCGTA52750.029.00669342
AATCTCG53200.028.93027740
TCGTATG56300.028.21586844
GAATCTC65100.023.50368939
TGAATCT76800.020.15743838
TTGAATC82500.018.6829237
GAGCGTC9300.018.38585316
AGCGTCG8300.017.3483717
AGAGCGT11700.016.72963515
CTTGAAT95600.016.2407736
ACTTGAA113550.014.12920935
CACTTGA118900.013.58796334
GCGTCGT7350.013.16259618
AAGAGCG15950.012.69506514
ACACTTG140600.011.47481333
CACACTT147600.010.90012532
GTCACAC147850.010.72950330
Produced by FastQC (version 0.11.2)