FastQCFastQC Report
Fri 5 Feb 2016
HGH7MBCXX_l02n01_plasmid-wt.3510000004113e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGH7MBCXX_l02n01_plasmid-wt.3510000004113e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13028039
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGC694720.5332498620859211TruSeq Adapter, Index 14 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCTCG106550.031.19016640
CGTATGC111950.030.6714645
CTCGTAT114250.029.9559943
ATCTCGT115550.028.89745741
TCGTATG122050.028.15195744
TCTCGTA123650.027.0418942
CCATCTC150100.022.29055839
TCCATCT157850.021.2104138
GTTCCAT179850.018.62887436
TTCCATC184350.018.23467837
TGCCCCG19400.013.8020715
AGTTCCA265800.013.46003345
CCCCGGG20300.013.1887387
GTGCCCC21400.012.5126864
CCCGGGT21800.012.2812568
CAGTTCC342550.010.37207144
CCGGGTA29350.010.3485159
GCCCCGG27550.09.55571656
GGTGCCC28500.09.4744443
GGGTAAC35550.09.23986311
Produced by FastQC (version 0.11.2)