FastQCFastQC Report
Fri 5 Feb 2016
HGH7MBCXX_l02n01_plasmid-d.35100000041107.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGH7MBCXX_l02n01_plasmid-d.35100000041107.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13552802
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCTCG29300.017.73952340
CTCGTAT37450.014.60029743
CGTATGC38250.014.17722345
ATCTCGT40350.012.99320741
TCGTATG43300.012.73162444
TCTCGTA43200.011.87580542
GGCTACA56750.011.53805845
ACATCTC46300.011.51767939
CGGCTAC64850.010.75616145
TGCCCCG21950.010.6608845
GCTACAT48550.010.6597236
GTGCCCC23300.010.1402484
CCCCGGG24350.09.6091617
TACATCT61300.09.13976238
CCCGGGT25750.09.0867228
ACGGCTA79300.08.85292944
CTACATC60150.08.75338837
CACGGCT81150.08.3461343
CCGGGTA33900.07.96402559
GCCCCGG30350.07.85853436
Produced by FastQC (version 0.11.2)