HGH7MBCXX_l01n02_t24-r.35200000041148.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HGH7MBCXX_l01n02_t24-r.35200000041148.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21347
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	81	0.37944441841945004	Illumina Single End PCR Primer 1 (100% over 51bp)
GTGACTGGAGTTCAGACGTGTGCTAGATCGGAAGAGCGTCGTGTAGGGAAA	56	0.2623319435986321	Illumina Single End PCR Primer 1 (100% over 27bp)
GTGACTGGAGTTCAGACGTGTGCTCAGATCGGAAGAGCGTCGTGTAGGGAA	33	0.15458846676347965	Illumina Single End PCR Primer 1 (100% over 26bp)
GTGACTGGAGTTCAGACGTGTGCAGATCGGAAGAGCGTCGTGTAGGGAAAG	24	0.11242797582798521	Illumina Single End PCR Primer 1 (100% over 28bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCATG	25	3.762969E-5	45.000004	22
ACGTCTG	25	3.762969E-5	45.000004	22
TGACTGG	85	0.0	45.000004	2
GAAGTGC	20	6.878337E-4	45.000004	7
TGCGACG	20	6.878337E-4	45.000004	11
AGGGCAT	20	6.878337E-4	45.000004	21
CGGAAGT	20	6.878337E-4	45.000004	5
AGAGCAT	25	3.762969E-5	45.000004	9
AGTGCGA	20	6.878337E-4	45.000004	9
GTGATTG	20	6.878337E-4	45.000004	1
AGGTCGC	20	6.878337E-4	45.000004	43
AGCACAC	40	6.2900654E-9	45.000004	17
AAGTGCG	20	6.878337E-4	45.000004	8
GTGCGAC	20	6.878337E-4	45.000004	10
ACTGGAG	100	0.0	45.000004	4
CGTCTGA	25	3.762969E-5	45.000004	23
CGATCTT	20	6.878337E-4	45.000004	29
ACACGTC	35	1.138651E-7	45.0	20
AGAGCAC	35	1.138651E-7	45.0	15
AGACGTG	75	0.0	45.0	14