FastQCFastQC Report
Fri 5 Feb 2016
HGH7MBCXX_l01n02_t24-hu.3520000004112e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGH7MBCXX_l01n02_t24-hu.3520000004112e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10801581
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG342850.3174072388106889Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC57300.033.61752344
GTCGCCG57950.033.39562245
GCACACG37800.029.8759518
AGAGCAC60400.029.7603315
TGGTCGC64100.029.7354143
CTCGGTG63600.029.43748538
GTGGTCG65150.029.4288842
GAGCACA61550.028.07003616
AGCACAC52450.026.42074417
TCTCGGT71100.026.30061537
TCGGTGG74400.025.64787539
CACACGT31200.025.37883819
GGTGGTC79500.024.3423141
CGGTGGT83550.022.91962640
ATCTCGG81750.022.8193536
CGGGGAC9550.022.3892331
ACACGTC26550.020.8444520
AAGAGCA107400.020.06758914
CGGGGCG3550.018.3860571
CGGGGAG9550.018.1470621
Produced by FastQC (version 0.11.2)