FastQCFastQC Report
Fri 5 Feb 2016
HGH7MBCXX_l01n02_t23-hu.35200000041111.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGH7MBCXX_l01n02_t23-hu.35200000041111.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12127733
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG417010.34384826908705857Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG68450.034.1925645
GGTCGCC68450.033.96227644
GCACACG62900.033.26102418
AGAGCAC91500.031.51962315
CACACGT52150.031.09978919
AGCACAC75600.030.97649217
GAGCACA90200.030.97550416
CTCGGTG73400.030.68812238
GTGGTCG75600.030.45255342
TGGTCGC78350.029.32625843
ACACGTC40850.028.85654620
TCTCGGT79000.028.45579137
TCGGTGG88050.025.73506439
ATCTCGG89050.025.24474736
GGTGGTC92500.024.7176741
CACGTCT31250.024.33216321
CGGTGGT96250.023.70651440
AAGAGCA143150.023.1807414
ACGTCTG27450.021.30881922
CGGGGAC9900.021.1442781
Produced by FastQC (version 0.11.2)