FastQCFastQC Report
Fri 5 Feb 2016
HGH7MBCXX_l01n02_t22-hu.352000000410f6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGH7MBCXX_l01n02_t22-hu.352000000410f6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11102333
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG54550.036.8676418
CACACGT51950.034.0340919
AGCACAC68350.033.0443917
AGAGCAC77800.032.06715415
ACACGTC42350.031.44667820
GAGCACA77350.031.38077516
CACGTCT34300.027.80879421
GGTCGCC16050.025.2379844
CGGGGAC8400.023.8483241
ACGTCTG27150.022.95271122
GTCGCCG18300.022.1349545
AAGAGCA120900.022.01316814
GATCGGA128050.021.9292418
AGATCGG127650.021.9099257
CGGGGAG9400.021.0718171
CGGGGCG5100.020.7431561
CGTCTGA23750.020.1769623
TCGGAAG138600.019.9335510
ATCGGAA141500.019.8443559
CAGATCG44750.019.6093046
Produced by FastQC (version 0.11.2)