FastQCFastQC Report
Fri 5 Feb 2016
HGH7MBCXX_l01n02_t21-hu.352000000410dc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGH7MBCXX_l01n02_t21-hu.352000000410dc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13866028
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG506770.36547596759504597Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG62800.034.99864618
GTCGCCG81550.034.16234645
GGTCGCC80950.034.1098944
TGGTCGC87100.031.31389443
AGCACAC81700.031.30795717
AGAGCAC96450.031.23196815
GAGCACA95150.030.47617516
GTGGTCG89850.030.45566442
CTCGGTG88000.029.96912238
CACACGT60550.029.64694819
TCTCGGT96950.027.20250537
ACACGTC46850.027.03400620
TCGGTGG104000.025.83403639
GGTGGTC108450.025.31446641
ATCTCGG111100.023.79893936
CGGTGGT115500.023.55438840
CACGTCT38300.022.90773621
AAGAGCA151250.022.5943914
CGGGGAC11600.020.955891
ACGTCTG32900.019.96720922
Produced by FastQC (version 0.11.2)