FastQCFastQC Report
Fri 5 Feb 2016
HGH7MBCXX_l01n02_t14-r.352000000410b2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGH7MBCXX_l01n02_t14-r.352000000410b2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9549692
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG100200.1049248499323329Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG43150.035.55626318
CACACGT42150.032.8760619
ACACGTC36550.032.19111320
AGCACAC52350.031.97192817
GAGCACA54950.031.60546516
AGAGCAC56000.031.53608915
CACGTCT33050.029.95088821
GGTCGCC20750.027.87100844
GTCGCCG22050.026.53398145
ACGTCTG28650.026.4632322
CGTCTGA24100.024.83197623
CTCGGTG27000.021.41826238
AAGAGCA86100.021.3743214
GTGGTCG27400.021.18882242
TGGTCGC28600.020.37846243
CGGGGAG6450.018.8452261
CGGGGTG6850.017.7447781
TCTCGGT33850.017.15046737
CGGGGAC6300.016.792861
TCGGTGG36100.016.64251539
Produced by FastQC (version 0.11.2)