FastQCFastQC Report
Fri 5 Feb 2016
HGH7MBCXX_l01n02_t14-hu.35200000041099.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGH7MBCXX_l01n02_t14-hu.35200000041099.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20030529
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG414950.20715878247648875Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG111550.037.12619418
CACACGT108550.034.60626219
AGCACAC135250.033.58112717
GAGCACA144450.032.85951216
AGAGCAC148500.032.79749715
ACACGTC94500.032.7561320
GGTCGCC75000.032.73644
GTCGCCG75600.032.65479745
CACGTCT80450.030.31221
TGGTCGC89800.027.39087743
ACGTCTG63400.027.32325222
GTGGTCG90450.027.24379742
CTCGGTG89400.027.05823538
AAGAGCA209650.024.42327914
CGTCTGA54850.024.2819523
TCGGTGG108900.022.56420339
TCTCGGT112150.021.74996237
GGTGGTC119700.020.7362141
CGGGGTG16450.020.2510191
CGGTGGT123600.020.11747240
Produced by FastQC (version 0.11.2)