FastQCFastQC Report
Fri 5 Feb 2016
HGH7MBCXX_l01n02_t13-hu.3520000004107f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGH7MBCXX_l01n02_t13-hu.3520000004107f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11368963
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG337650.2969927864133255Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG97550.039.34045418
CACACGT92450.037.7366919
ACACGTC81650.037.66269320
AGCACAC110800.037.1534317
GAGCACA117900.036.61413616
CACGTCT71200.036.271621
AGAGCAC121400.036.2270415
GGTCGCC55650.033.92799844
GTCGCCG56100.033.73622545
ACGTCTG56200.033.46537822
CGTCTGA46150.031.39420723
GTGGTCG62950.029.67192642
AAGAGCA163950.028.22570814
CTCGGTG65500.028.06875438
TGGTCGC68300.027.41359343
TCGGTGG76200.024.24418639
GGTGGTC78250.024.0416541
TCTCGGT76950.024.03839937
CGGTGGT84450.022.11623840
CGGGGAG9100.021.5183071
Produced by FastQC (version 0.11.2)