FastQCFastQC Report
Fri 5 Feb 2016
HGH7MBCXX_l01n02_t12-hu.35200000041056.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGH7MBCXX_l01n02_t12-hu.35200000041056.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9750366
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG201550.20671018913546424Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG75350.039.9748918
CACACGT72250.038.13909519
ACACGTC61000.038.1329820
CACGTCT51250.036.2136521
AGCACAC88450.036.19070417
GAGCACA96550.035.08832616
AGAGCAC101000.034.65704715
GGTCGCC36600.033.44887544
GTCGCCG37150.032.83234845
ACGTCTG41650.031.49014722
CGTCTGA33050.031.17626423
AAGAGCA129800.028.3732114
GTGGTCG43400.028.15618742
TGGTCGC44650.027.16633243
CTCGGTG45000.026.70348438
GGTGGTC54650.022.7299941
TCGGTGG54800.022.29681639
TCTCGGT54200.022.04623637
CGGTGGT58000.021.14478540
ATCTCGG63050.019.05908636
Produced by FastQC (version 0.11.2)