FastQCFastQC Report
Fri 5 Feb 2016
HGH7MBCXX_l01n02_t11-hu.3520000004103c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGH7MBCXX_l01n02_t11-hu.3520000004103c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15124863
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG180590.11939942860970047Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG111300.038.1394718
CACACGT105400.036.53695319
ACACGTC89250.035.86779420
AGCACAC128400.035.5129417
AGAGCAC140150.034.71933415
GAGCACA137950.034.6362416
CACGTCT76350.033.56049721
ACGTCTG61950.030.46833622
GGTCGCC36200.029.8394944
GTCGCCG37500.029.10501145
CGTCTGA50800.028.60937523
AAGAGCA189100.026.77973714
CTCGGTG43800.024.09478438
GTGGTCG46000.023.53138242
TGGTCGC47700.022.45684643
CGGGGAG13000.020.0837691
TCGGTGG56550.019.09972839
TCTCGGT59650.017.9187437
GGTGGTC63150.017.35390341
CGGGGAC12150.017.0428471
Produced by FastQC (version 0.11.2)