FastQCFastQC Report
Fri 5 Feb 2016
HGH7MBCXX_l01n01_t24-r.3510000004114b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGH7MBCXX_l01n01_t24-r.3510000004114b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21347
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC6933.246357802033072TruSeq Adapter, Index 14 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGC1000.4684498992832717TruSeq Adapter, Index 14 (98% over 50bp)
AGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTC680.3185459315126247TruSeq Adapter, Index 14 (96% over 29bp)
GAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACT350.16395746474914508TruSeq Adapter, Index 14 (96% over 30bp)
GCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCC250.11711247482081792Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTTC600.00630781318.74999843
CAGTCAC3301.8189894E-1212.95454528
AGTCACA3151.4551915E-1112.85714329
TCACAGA2652.7794158E-912.73584931
CCAGTCA3403.6379788E-1212.5735327
CACAGAT2703.6743586E-912.50000132
GTCACAG3252.5465852E-1112.46153730
TCCAGTC3455.456968E-1212.39130426
ACAGATC2754.829417E-912.27272733
GAACTCC4050.012.22222122
CTCCAGT3507.2759576E-1212.21428625
TGAACTC4250.012.17647121
ACGTCTG4300.012.03488416
GTCTGAA4300.012.03488418
CGTCTGA4350.011.89655217
AACTCCA4000.011.81250123
CACGTCT4400.011.76136315
TCTGAAC4400.011.76136319
CTGAACT4450.011.62921320
ACACGTC4600.011.25000114
Produced by FastQC (version 0.11.2)