FastQCFastQC Report
Fri 5 Feb 2016
HGH7MBCXX_l01n01_t24-hu.35100000041121.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGH7MBCXX_l01n01_t24-hu.35100000041121.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10801581
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC383520.3550591343989366TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCTCG63550.028.61015140
CTCGTAT72300.026.29860543
CGTATGC75200.025.734145
ATCTCGT75900.024.37050241
TCGTATG82400.023.51192744
TCTCGTA78600.023.38911442
ACATCTC80050.022.9660939
GCTACAT92700.019.87972636
CTACATC96000.019.3140937
TACATCT95900.019.31076838
GGCTACA117700.016.1735835
CGGCTAC133250.014.21847834
ACGGCTA157450.012.03310433
CACGGCT164450.011.47985432
TCACGGC172250.010.92082131
CCCTACG19150.010.5754340
GTCACGG179450.010.53280530
TACGGCC19450.010.18069443
CTACGGC19450.010.18069442
AGTCACG185850.010.13377329
Produced by FastQC (version 0.11.2)