FastQCFastQC Report
Fri 5 Feb 2016
HGH7MBCXX_l01n01_t23-hu.35100000041114.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGH7MBCXX_l01n01_t23-hu.35100000041114.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12127733
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC468480.3862881875780082TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG76400.028.51064340
CTCGTAT82500.027.65656743
CGTATGC83250.027.54312145
ATCTCGT85200.025.83052641
TCGTATG91050.025.05928644
TCTCGTA89100.024.97657442
ATATCTC109700.020.24599539
AATATCT142550.015.75397338
CAATATC143200.015.74532137
CCAATAT143050.015.72972136
CGCCAAT150050.015.34107134
GCCAATA149650.015.33671735
CACGCCA150250.015.33562732
GTCACGC148750.015.29361930
TCACGCC150050.015.25109531
ACGCCAA157050.014.65729233
AGTCACG155850.014.59689129
CAGTCAC181450.013.169826528
CCAGTCA197750.012.174827
TCCAGTC203450.012.01080426
Produced by FastQC (version 0.11.2)