FastQCFastQC Report
Fri 5 Feb 2016
HGH7MBCXX_l01n01_t22-hu.351000000410f9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGH7MBCXX_l01n01_t22-hu.351000000410f9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11102333
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTCG23200.015.32494140
CGTATGC29100.013.29993545
CTCGTAT27750.013.05506243
ATCTCGT30550.011.93266541
AGTGATC31800.011.88792337
TCGTATG34300.011.28341544
GTGATCT33550.011.066627538
TGATCTC37800.09.94151339
CCCCGGG21550.09.7092397
TGCCCCG22950.09.3142355
GTGCCCC23450.09.2116744
TCTCGTA38750.09.17490242
CCCGGGT23150.09.1353758
CGGACGG5101.0786607E-98.82222319
GGACGGG5454.110916E-108.66851920
CAGTGAT50650.08.08541136
GCCCCGG26800.07.9767216
CCGGGTA30200.07.8967519
CACACAG48400.07.8104532
GTCACAC48600.07.68570930
Produced by FastQC (version 0.11.2)