FastQCFastQC Report
Fri 5 Feb 2016
HGH7MBCXX_l01n01_t21-hu.351000000410df.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGH7MBCXX_l01n01_t21-hu.351000000410df.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13866028
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC535400.38612355319057484TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC97500.027.3473745
GATCTCG90550.027.33526840
CTCGTAT96650.027.00605443
CACGATC96700.025.52696837
TCTCGTA98550.025.29811342
ATCTCGT99500.024.96710841
TCGTATG107150.024.84209444
CGATCTC102000.024.2007439
ACGATCT103400.023.89466338
TCACGAT121650.020.6056336
ATCACGA169750.015.66836435
CATCACG192400.013.76520434
ACATCAC240300.011.18051533
GTCACAT244700.010.85073930
TCACATC247050.010.78395731
AGTCACA266000.010.67551829
CACATCA252600.010.64500432
CAGTCAC271350.010.53959428
CCAGTCA286700.09.98293327
TCCAGTC295300.09.8598726
Produced by FastQC (version 0.11.2)