FastQCFastQC Report
Fri 5 Feb 2016
HGH7MBCXX_l01n01_t14-r.351000000410b5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGH7MBCXX_l01n01_t14-r.351000000410b5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9549692
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC128760.1348315736256206TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC32400.019.72329745
CTCGTAT32450.019.48498543
AATCTCG33600.018.34959840
ATCTCGT34150.017.98827241
TCGTATG37050.017.6121144
TCTCGTA39000.015.69324342
CCCCGGG16250.014.8141757
TGCCCCG15850.014.7646755
GTGCCCC16600.014.0975974
TAATCTC46600.013.66526439
CCCGGGT17950.013.6618378
GTAATCT46900.013.3856638
TGTAATC47750.013.10025937
GCCCCGG19450.012.1478916
CCGGGTA24050.011.8805279
GGTGCCC22150.010.6669973
CGGGTAA30950.010.39495110
CTTGTAA97900.010.22782945
CCTTGTA98200.010.01316944
GGGTAAC29400.09.6420711
Produced by FastQC (version 0.11.2)