FastQCFastQC Report
Fri 5 Feb 2016
HGH7MBCXX_l01n01_t14-hu.3510000004109c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGH7MBCXX_l01n01_t14-hu.3510000004109c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20030529
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC500280.24975875574728953TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC90750.028.24156845
TATCTCG87750.028.1566540
CTCGTAT99500.025.73552743
TCGTATG104350.024.75452844
ATCTCGT101350.024.37841441
TCTCGTA108150.023.0529842
GCTTATC121200.020.23691237
TTATCTC135600.018.51954339
CTTATCT140350.017.84444638
TAGCTTA243800.015.01639145
AGCTTAT180650.013.76355436
CTAGCTT343750.011.481545
CCCCGGG33850.011.4314497
TGCCCCG33750.011.2674245
CCCGGGT37000.010.4582318
GTGCCCC37350.010.18145754
ACTAGCT397000.09.90731844
GCCCCGG38650.09.8974696
CCGGGTA48800.09.727349
CACTAGC423400.09.2791343
Produced by FastQC (version 0.11.2)