FastQCFastQC Report
Fri 5 Feb 2016
HGH7MBCXX_l01n01_t13-hu.35100000041072.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGH7MBCXX_l01n01_t13-hu.35100000041072.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11368963
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC371870.3270922774574955TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC67750.028.4637745
GATCTCG63350.028.13229640
CTCGTAT68250.027.76057243
ATCTCGT66650.027.01008841
TCGTATG73400.026.4254644
AGATCTC73450.024.41726139
TCTCGTA75750.024.12083642
CAGATCT91050.019.99396938
TCAGATC107050.016.8794937
ATCAGAT131450.013.93397936
TGCCCCG36600.013.8327045
GTGCCCC38400.013.242954
CCCCGGG39550.012.6850847
GATCAGA154000.012.53665635
CCCGGGT40800.012.1861658
GCCCCGG44200.011.5566556
CCGGGTA47800.011.5311639
CGATCAG172700.010.95807134
GGTGCCC47300.010.7990053
CAGGTGC48600.010.69675351
Produced by FastQC (version 0.11.2)