FastQCFastQC Report
Fri 5 Feb 2016
HGH7MBCXX_l01n01_t12-hu.35100000041059.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGH7MBCXX_l01n01_t12-hu.35100000041059.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9750366
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC224220.22996059840215227TruSeq Adapter, Index 8 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC45500.027.79191645
CTCGTAT46650.026.62474443
TCGTATG50900.024.88741144
ATCTCGT49100.024.47247141
TCTCGTA51800.023.67362442
AATCTCG52100.023.02047340
GAATCTC66900.018.06247939
TGAATCT83350.014.57865138
TTGAATC89050.013.44333237
TGCCCCG21250.013.0243295
CCCCGGG21500.012.7659347
GTGCCCC22200.012.3656854
CCCGGGT23000.011.8355578
CTTGAAT107000.011.52430736
CCGGGTA29000.010.6280549
CACTTGA121850.010.19416534
GGTGCCC27850.09.9380263
ACTTGAA129200.09.84030235
GTAACCC31450.09.01296613
GTCACAC139200.08.93972230
Produced by FastQC (version 0.11.2)