FastQCFastQC Report
Fri 5 Feb 2016
HGH7MBCXX_l01n01_t11-hu.3510000004103f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGH7MBCXX_l01n01_t11-hu.3510000004103f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15124863
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC207840.13741612072783732TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCTCG44250.023.95181540
CGTATGC52250.021.2747445
CTCGTAT54000.020.41846343
TCGTATG58050.019.34253144
ATCTCGT55050.019.25326741
TCTCGTA59900.017.99407242
TCATCTC79250.013.99854139
GATCATC83600.013.16230637
CAGATCA130000.012.84352345
TGCCCCG31400.010.82076555
CCCCGGG31250.010.7989517
CCCGGGT33450.010.2904818
GTGCCCC33350.010.120634
AGATCAT112950.09.94088436
CCGGGTA44800.09.2401739
ATCATCT128100.09.16960838
GCCCCGG38700.08.8382226
CCAGATC191000.08.80039744
GGTGCCC39900.08.5722963
GTAACCC43650.08.03997713
Produced by FastQC (version 0.11.2)