FastQCFastQC Report
Tue 6 Sep 2022
HGGTNBGXM_n01_DO31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGTNBGXM_n01_DO31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7711268
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC102121713.243178683453877TruSeq Adapter, Index 8 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATATCGTATGC204420.26509258918247947TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATTC148090.19204364314662645TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTATGC109830.14242793792149358TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGATGC83680.10851652413066179TruSeq Adapter, Index 8 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)