FastQCFastQC Report
Tue 10 Jan 2017
HGGNWBGX2_n01_w9_umi.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGNWBGX2_n01_w9_umi.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15313959
Sequences flagged as poor quality0
Sequence length76
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTCCTCTCCGTTTCAAAGGCCTGATTTTATGCAGGCCACCATCGAAAG936710.6116706986090272No Hit
AGTTCCTCTCGCATTTCACTGGGCCAGCATCAGTTTTGGTGGCAGGATAA884790.577766990234204No Hit
AGTTCCTGTTATCTTTTCTTCTTAACAGCTTATCACCCCGGAATTGGTTT411020.2683956513139418No Hit
AGTTCCTCTGCGACGTAAGTCAAGGATGCTGGCATAATGGTTATATGCCG327170.21364168468780673No Hit
AGTTCCTCTTCGGCGCCAGTGAAATACCACTACCTTTATAGTTTCTTTAC300260.19606948144500058No Hit
AGTTCCTCTTTTCTTCTTAACAGCTTATCACCCCGGAATTGGTTTATCCG295440.19292202623763063No Hit
AGTTCCTGGTTCCACGTCAACAGCAGTTGGACGTGGGTTAGTCGATCCTA263200.17186933829455858No Hit
AGTTCCTGTGATAACAACCTTCTTGGCACCAGCGTCAATGTGCTTTTGAG240550.1570789108159425No Hit
AGTTCCTCTTGCTGTCTCTAGCAGCTTCTAAAGCAGCGACGGCAGTAGAA220110.1437316111398757No Hit
AGTTCCTGACAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTG211170.1378938000291107No Hit
AGTTCCTCTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCT206950.13513814422514778No Hit
AGTTCCTGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCA203490.13287876766550047No Hit
AGTTCCTGTTCCACGTCAACAGCAGTTGGACGTGGGTTAGTCGATCCTAA196370.12822941474507016No Hit
AGTTCCTGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTA174280.11380466670963399No Hit
AGTTCCTGAAGAAATTAAAAGAATTTCTACTAAATCCCAATTGTTATATA166720.10886799422670519No Hit
AGTTCCTGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGC166600.10878963434602378No Hit
AGTTCCTATCTTTTCTTCTTAACAGCTTATCACCCCGGAATTGGTTTATC164920.10769259601648405No Hit
AGTTCCTGCAACTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGA158370.10341545252929042No Hit
AGTTCCTCAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTGAC157670.10295835322531556No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTCCG5689600.069.8743061
GTTCCGC2005250.069.74062
AGTTCCT7870200.069.675961
GTTCCTA1820400.069.5325552
GTTCCGA1363000.069.51892
TTCCGCT985500.069.273043
GTTCCGG1841150.068.925732
GTTCCGT781400.068.8491742
AGTTCCC697450.068.7638241
GTTCCTC2673500.068.641192
TCCTAGC212900.068.34594
CCGCTCG156200.068.303355
TTCCGAA423100.067.989613
CGCTCGC114650.067.9609156
TTCCGAT425950.067.90453
GTTCCCG317650.067.805452
TTCCGGC432300.067.465773
TTCCTAA569150.067.3144763
TCCTGTC264650.067.215734
CCTCGCG57450.067.2037665
Produced by FastQC (version 0.11.2)