FastQCFastQC Report
Tue 10 Jan 2017
HGGNWBGX2_n01_w8_umi.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGNWBGX2_n01_w8_umi.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18396422
Sequences flagged as poor quality0
Sequence length76
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCAGTCTCGCATTTCACTGGGCCAGCATCAGTTTTGGTGGCAGGATAA736810.4005181007480694No Hit
GATCAGTCTCCGTTTCAAAGGCCTGATTTTATGCAGGCCACCATCGAAAG643290.3496821284051866No Hit
GATCAGTCTTGCTGTCTCTAGCAGCTTCTAAAGCAGCGACGGCAGTAGAA529450.2878005299073918No Hit
GATCAGTAAAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACT442950.24078051699401112No Hit
GATCAGTGACAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTG368480.20029981917135845No Hit
GATCAGTGTGATAACAACCTTCTTGGCACCAGCGTCAATGTGCTTTTGAG333030.1810297676363371No Hit
GATCAGTGTTATCTTTTCTTCTTAACAGCTTATCACCCCGGAATTGGTTT312170.1696906061406941No Hit
GATCAGTGAAGAAATTAAAAGAATTTCTACTAAATCCCAATTGTTATATA299960.16305344593638915No Hit
GATCAGTCTGCGACGTAAGTCAAGGATGCTGGCATAATGGTTATATGCCG264970.14403344302495344No Hit
GATCAGTCAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTGAC231500.12583968773927887No Hit
GATCAGTCTTCGGCGCCAGTGAAATACCACTACCTTTATAGTTTCTTTAC226010.12285541177518107No Hit
GATCAGTCTTTTCTTCTTAACAGCTTATCACCCCGGAATTGGTTTATCCG218330.11868068692923003No Hit
GATCAGTAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTAT210250.1142885284975524No Hit
GATCAGTAAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTA201430.10949411793228052No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCAGT11634700.069.85651
ATCAGTC4108200.069.3865362
TCAGTCG740150.068.7765663
ATCAGTG3561350.068.714212
TCAGTGC748000.068.354273
TCAGTCC612750.067.978233
CAGTCGC159900.067.5746464
ATCAGTA3079350.067.3647842
CAGTGCG140350.067.1114964
CAGTGCA189500.066.9382864
ATCAGGA934650.066.8879852
GATCAGG3526550.066.743231
GTCTCGC110050.066.537446
CAGTCTG373350.066.498124
TCAGTCT2001900.066.1769563
CAGTCCG150000.066.037524
TCAGGCG223050.066.0044563
TCAGTCA917250.065.93823
TCAGTGT1428850.065.919983
TCAGTAT862000.065.6612243
Produced by FastQC (version 0.11.2)