FastQCFastQC Report
Tue 10 Jan 2017
HGGNWBGX2_n01_w7_umi.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGNWBGX2_n01_w7_umi.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15671413
Sequences flagged as poor quality0
Sequence length76
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGATCTCTTGCTGTCTCTAGCAGCTTCTAAAGCAGCGACGGCAGTAGAA408220.26048704095795316No Hit
CAGATCTCTCCGTTTCAAAGGCCTGATTTTATGCAGGCCACCATCGAAAG403010.25716251623258224No Hit
CAGATCTAAAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACT304750.19446236277481807No Hit
CAGATCTCTCGCATTTCACTGGGCCAGCATCAGTTTTGGTGGCAGGATAA255930.16331009845761835No Hit
CAGATCTGACAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTG248950.15885612867199658No Hit
CAGATCTGTCGACATGTAGTTAAACCACTATTCAGTCGGAACAATTGGTA245050.15636752091212197No Hit
CAGATCTGTGATAACAACCTTCTTGGCACCAGCGTCAATGTGCTTTTGAG232250.1481997826233027No Hit
CAGATCTATGAACAACAATTGTTGTAGTCGCAACTACGGTAATTGGTCCT223200.14242493641128595No Hit
CAGATCTATTTGATGGAAGCAAGTACTTTTTAGATACCCAGTAAAAGTCT216480.13813687380965584No Hit
CAGATCTGTTATCTTTTCTTCTTAACAGCTTATCACCCCGGAATTGGTTT200120.1276974833092587No Hit
CAGATCTCAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTGAC192540.12286065079134854No Hit
CAGATCTGAAGAAATTAAAAGAATTTCTACTAAATCCCAATTGTTATATA191700.12232464296614479No Hit
CAGATCTCAAAGGTTATGGGACTCATCAACCAAGTTGGATTCAGTGGCTC182400.11639027061567453No Hit
CAGATCTCTTTAAATGGGTCAGGGAATTCGGTAGGAGATTCTGGAGGTAG166610.1063145997109514No Hit
CAGATCTAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTAT157410.10044403781586256No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGATCG2739850.069.4944151
AGATCTA2663650.069.346112
AGATCGC887700.068.774222
AGATCTC3929050.068.47932
GATCTGT1437650.068.2072143
CAGATCT11787250.068.1878361
GATCTCC621250.068.139113
GATCTAT902800.067.813463
GATCGCC142850.067.798763
GATCGCT415800.067.714313
GATCTAG730500.067.680123
GATCGAG182350.067.527853
GATCGGC172750.067.430773
CAGATCA841250.067.334091
GATCTAA752100.066.951043
GATCTCA830100.066.90483
GATCGCA184300.066.718413
GATCGGT313500.066.587543
AGATCGA707750.066.453542
GATCTCG781800.066.3909763
Produced by FastQC (version 0.11.2)