FastQCFastQC Report
Tue 10 Jan 2017
HGGNWBGX2_n01_w6_umi.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGNWBGX2_n01_w6_umi.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11927721
Sequences flagged as poor quality0
Sequence length76
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGTGTGAAGAAATTAAAAGAATTTCTACTAAATCCCAATTGTTATATA323790.27146007187793875No Hit
ACAGTGTATTTGATGGAAGCAAGTACTTTTTAGATACCCAGTAAAAGTCT224680.18836792040994252No Hit
ACAGTGTCTTGCTGTCTCTAGCAGCTTCTAAAGCAGCGACGGCAGTAGAA163180.13680735825393636No Hit
ACAGTGTTGAAATTGTAGGTCACCAATAGCGTTAATTGGTATATTTCTTT158930.13324422997486274No Hit
ACAGTGTAACAGTTTCAACGGTGGAAGATGGGGCAGCAGTGGTGGTAGCT144360.121028987851074No Hit
ACAGTGTGTGATAACAACCTTCTTGGCACCAGCGTCAATGTGCTTTTGAG135200.11334939843076478No Hit
ACAGTGTCCACCGTTCAAAACGTTCAAGAATGGAACTGGCAAAACGTATG120860.10132698442560821No Hit
ACAGTGTGTAGCAATGTCAGTGATCATAGCAGTGGTTTCAGAAATGTTGG120120.10070658091348716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTGT6280700.069.619071
CAGTGCG334400.069.2937552
CAGTGTG2399950.069.260252
CAGTGGG856400.068.7466662
ACAGTGC616150.068.399381
CAGTGAG558600.068.100622
CAGTGTA1965950.067.9366462
ACAGTGG2398100.067.7165151
AGTGTGC509950.067.492473
AGTGTGT940150.067.297993
GCAGTGC224950.067.264061
CAGTGCA171550.067.087782
AGTGGGT343700.066.7152253
AGTGTCG405750.065.734493
AGTGCGG85500.065.5833053
AGTGTAG569300.065.5778663
AGTGGGG216950.065.5355763
CAGTGTC2073400.065.521722
AGTGTGG598900.065.22393
AGTGCGT137850.065.2056353